Recombinant Mouse Anti-AAV VP1 Antibody (A20)
CAT#: PABL-390
Recombinant Mouse Antibody (A20) is capable of binding to AAV VP1 , expressed in Chinese Hamster Ovary cells (CHO). A20 is a monoclonal antibody which neutralizes AAV-2 and AAV-3B subsequent to primary receptor binding.
Published Data
Tested Data
ICC
Figure 1 Time course of AAV infection using A20 immunocytochemistry.
HeLa cells were infected with wild-type AAV at a MOI of 10,000 particles per cell for 10 min at 37°C, washed to remove unadsorbed virus, and incubated at 37°C. Viral particles were detected with A20 monoclonal antibody.
Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.
IF
Figure 2 Time course of AAV infection in the presence of Ad coinfection.
HeLa cells were infected with wild-typeAAV as in panel A and incubated at 37°C for 10 min. Samples were then stained with A20 antibody, which stains nucleic acid in the nucleus.
Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.
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SDS-PAGE
Figure 1 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in SDS-PAGE
SDS-PAGE analysis of PABL-390 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 25 kDa and 50 kDa, respectively.
SEC-HPLC
Figure 2 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in SEC-HPLC
The purity of PABL-390 was 99.7796% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in DB
Dot Blot analysis of PABL-390 was performed by coating with AAV2/2 virus particles (6.78E+12).
PABL-390 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
ELISA
Figure 4 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in ELISA
ELISA analysis of PABL-390 was performed by coating with AAV2/2 virus particles (6.78E+12). Then blocked with BSA and incubated with PABL-390. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- AAV Capsid protein VP1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native AAV VP1
- Species Reactivity
- AAV
- Clone
- A20
- Applications
- ICC, IF
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Immunocytochemistry and Immunofluorescence.
Target
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Optimal for Long-Term Projects
The stability and performance consistency of this antibody makes it ideal for long-term research projects. It's a reliable reagent in our lab.
User-Friendly Protocols
The provided datasheet offered detailed and user-friendly protocols. Even for someone relatively new to antibody research, the step-by-step instructions were clear and easy to follow.
Great for Multiplexing
We integrated this recombinant antibody into our multiplex assays, and it performed exceptionally well. The signal quality was high, and there was no cross-reactivity with other antibodies.
Q&As
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What type of secondary detection methods can I use?
A: Secondary detection methods include fluorescent labeling, enzyme-linked assays (such as ELISA), and chemiluminescence. The choice depends on the specific experimental setup.
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How should I address non-specific binding in my assay?
A: Non-specific binding can be minimized by optimizing blocking conditions and using stringent wash steps. Reducing antibody concentrations may also help.
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Can this antibody detect low-abundance proteins?
A: Yes, but detecting low-abundance proteins may require optimization of assay conditions, including increased antibody concentrations or enhanced detection systems.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-390, RRID: AB_3111652)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| PFBL-131 | Recombinant Mouse Anti-HEV-C VP1 Antibody Fab Fragment (C3) | WB, Neut, FuncS | Fab |
| PFBZ-047 | Recombinant Mouse Anti-FMDV VP1 pentadecapeptide (YTTSTRGDLAHVTAT) Antibody Fab Fragment (4C4) | Neut, ELISA | IgG |
| PFBW-047 | Recombinant Mouse Anti-FMDV-C-S8c1 VP1 Antibody Fab Fragment (SD6) | Neut | IgG |
| PFBW-074 | Recombinant Mouse Anti-HRV14 VP1 Antibody Fab Fragment (17-IA) | Neut | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1333 | PE-A*01:01/Virus VP1 (SADNNNSEY) MHC Tetramer | FCM, IHC | |
| MHC-CN1334 | APC-A*01:01/Virus VP1 (SADNNNSEY) MHC Tetramer | FCM, IHC | |
| MHC-CN1335 | FITC-A*01:01/Virus VP1 (SADNNNSEY) MHC Tetramer | FCM, IHC | |
| MHC-CN1336 | A*01:01/Virus VP1 (SADNNNSEY) MHC Tetramer | FCM, IHC | |
| MHC-CN1337 | PE-A*02:01/Virus VP1 (ILMWEAVTL) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
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| HPAB-0506-YJ-VHH | Llama Anti-VP1 Recombinant Antibody (HPAB-0506-YJ-VHH) | ELISA, IF, Inhib, WB | Llama VHH |
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